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Search for "emission-based dichroism" in Full Text gives 1 result(s) in Beilstein Journal of Organic Chemistry.

Polarization spectroscopy methods in the determination of interactions of small molecules with nucleic acids – tutorial

Tamara Šmidlehner,
Ivo Piantanida and
Gennaro Pescitelli

Beilstein J. Org. Chem. 2018, 14, 84–105, doi:10.3762/bjoc.14.5

between nucleic acids and small ligands. This tutorial aims to help researchers entering the research field to organize experiments accurately and to interpret the obtained data reliably. Keywords: circular dichroism; emission-based dichroism; groove binding; intercalation; linear dichroism; non-covalent

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Graphical Abstract

Figure 1: Plane polarized light (PP, cyan) as the sum of the left and right circularly polarized light (L-CP,...

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Figure 2: Schematic representation of ligand binding modes to DNA or RNA (left to right: DNA minor groove bin...

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Scheme 1: Principal electronic transitions of nucleobases (uracil is similar to thymine, Thy). The arrows dep...

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Figure 3: Exciton coupling mechanism in its degenerate (top) and non-degenerate version (bottom). a) Chiralit...

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Figure 4: ECD and UV (inset) spectra of poly(dG-dC)–poly(dG-dC) in the B and Z-form, obtained in 59% and 67% ...

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Figure 5: Schematic illustration of an ECD instrument. Legend: S, source; M, monochromator (wavelength select...

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Figure 6: Exciton coupling in the G-quadruplex [d(TG₄T)]₄. For this sequence, the quadruplex is formed by fou...

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Figure 7: Empirical analysis of ECD and flow LD spectra to establish the preferred orientation of a ligand bo...

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Figure 8: CD titration of poly rA-poly rU (left) and poly rU (right) (c(polynucleotide) = 2.0 × 10⁻⁵ mol dm⁻³...

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Figure 9: a) Schematic diagram of a flow LD instrument. b) Definition of the optical factor relating the dire...

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Figure 10: LD spectrum of calf thymus (ct) DNA (c = 2 × 10⁻⁴ M), pH 7, sodium cacodylate buffer, I = 0.05 M.

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Figure 11: Schematic illustration of an FDCD a) and a CPL b) instrument. Legend: S, source; M, monochromator (...

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Figure 12: Minimal model to be considered to simulate the ICD spectrum of an intercalated ligand. ICD will be ...

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Figure 13: Schematic representation of the main steps for calculating the ICD spectrum of a ligand bound to a ...

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Published 08 Jan 2018

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